Notes on Clancy-Rudy 1999, 2001 and 2002 Models (29th August 2006) :-

Following conversation with CC by phone:

- Tried with second order Runge-Kutta integrator instead of forward euler (use second order or implicit methods)

- CC thought L-type calcium current may be a problem but checked equations and could not find any error

NB: 14/4/08 If change the PCa  to 0.0025 in the R56Q mutation then get the EADs.
See model faber_rudy_with_clancy_rudy_ikr2001_Mcell_R56Q_model in folder AlteredModels of CellMLModelsCurated.

- Make sure at steady state... could not get models to steady state as rpd continually dropped as Nai continually rises. Hence tried clamping Nai and Ki. This may be okay for now but not an ideal solution!

- Tried reducing time-step 0.0001 ms

- How important are values of Nai or Ki as may get eads depending on concs. Reducing Nai to 7mM gave eads in CR2002McellInsD at 1.0 CL but same in WT! 

- Tried decreasing value of Ko... also did not help!

Summary of results:

- Herg mutations seem to work okay (T474I and R56Q in M cell and N629D in epi cell) but not (N629D in M cell) as does not give EAD.

- INaKPQ mutant (CR99) does not work okay... cell fails to repolarise.

- INa 1795insD seems to work okay in M cell at 0.3 and 0.85 CL but not 1.0 CL as do not get EAD.

- INa 1795insD does not work okay in epi cell at 0.3 CL.

Additional notes as of 11th September 2007:-
Error noticed missing “2*” in INaCa in dCai/dt… corrected and may make this a little better but still no EADs